A good tool to evaluate RNA-Seq library complexity by estimating PCR duplicates / biological duplicaFor RNA-Seq, it has been challenging to distinguish PCR duplicates from biological duplicates. Ghost found a tool that tackles this and...
Be careful of the poly-G sequence from NextSeq runWe may occasionally find unexpected amount of poly-G reads in raw data generated from NextSeq. See FASTQC figure below as a typical...
DNA fragment size, insert size and pre-mergingDuring the standard library preparation, we usually use physical or enzymatic methods to fragment DNA. How different fragment size...